anti sirtuin type 1 sirt1 (Cell Signaling Technology Inc)
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Anti Sirtuin Type 1 Sirt1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+sirtuin+1/pm41761283-106-31-36
Average 86 stars, based on 1 article reviews
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Incubation:Article Title: Effects of American wild ginseng and Korean cultivated wild ginseng pharmacopuncture extracts on the regulation of C2C12 myoblasts differentiation through AMPK and PI3K/Akt/mTOR signaling pathway Article Snippet: .. The membranes underwent incubation with primary antibodies anti-peroxisome proliferator-activated receptor-γ coactivator-1-α (PGC-1α, 1:1,000; cat. no. NBP1-04676; Bioss Antibodies), anti-mitochondrial transcription factor A (TFAM, 1:1,000; cat. no. PA5-68789; Thermo Fisher Scientific, Inc.), anti-nuclear respiratory factor-1 (NRF-1, 1:1,000; cat. no. 69432s; Cell Signaling Technology, Inc.), anti-phospho-AMPK (1:500; cat. no. 44-11509; Thermo Fisher Scientific, Inc.), anti-AMPK (1:500; cat. no. AHO1332; Thermo Fisher Scientific, Inc.), Article Title: Effects of American wild ginseng and Korean cultivated wild ginseng pharmacopuncture extracts on the regulation of C2C12 myoblasts differentiation through AMPK and PI3K/Akt/mTOR signaling pathway. Article Snippet: .. The membranes underwent incubation with primary antibodies anti‐peroxisome proliferator‐activated receptor‐γ coactivator‐1‐α (PGc‐1α, 1:1,000; cat. no. nBP1‐04676; Bioss antibodies), anti‐mitochondrial transcription factor a (TFaM, 1:1,000; cat. no. Pa5‐68789; Thermo Fisher Scientific, inc.), anti‐nuclear respiratory factor‐1 (nrF‐1, 1:1,000; cat. no. 69432s; cell Signaling Technology, inc.), anti‐phospho‐aMPK (1:500; cat. no. 44‐11509; Thermo Fisher Scientific, Inc.), anti‐AMPK (1:500; cat. no. AHO1332; Thermo Fisher Scientific, Inc.), Article Title: Exposure to dim light at night alters daily rhythms of glucose and lipid metabolism in rats. Article Snippet: Total proteins (40 or 80 μg) were separated by SDS-PAGE electrophoresis at 60 V for 20 min and then at 90 V for 80 min. Proteins were transferred to a nitrocellulose membrane using the vertical apparatus (Bio-Rad Laboratories, Hercules, CA, United States) at 230 mA for 75 min. Membranes were blocked by 1% fat-free milk or 5% bovine serum albumin (BSA) in TBS containing Tween 20 (TBS-T) for 1 h at room temperature. .. Subsequently, membranes were incubated with a primary antibody overnight at 4°C: anti-AMP-activated protein kinase α (2603, AMPKα; Cell Signaling Technology, Danvers, MA, United States, 1:1,000 dilution in 1% fat-free milk in TBST), anti-phospho-AMPKα (4188, Cell Signaling Technology, Danvers, MA, United States, 1:1,000 dilution in 1% fat-free milk in TBS-T), Article Title: Exposure to dim light at night alters daily rhythms of glucose and lipid metabolism in rats Article Snippet: Total proteins (40 or 80 μg) were separated by SDS-PAGE electrophoresis at 60 V for 20 min and then at 90 V for 80 min. Proteins were transferred to a nitrocellulose membrane using the vertical apparatus (Bio-Rad Laboratories, Hercules, CA, United States) at 230 mA for 75 min. Membranes were blocked by 1% fat-free milk or 5% bovine serum albumin (BSA) in TBS containing Tween 20 (TBS-T) for 1 h at room temperature. .. Subsequently, membranes were incubated with a primary antibody overnight at 4°C: anti-AMP-activated protein kinase α (2603, AMPKα; Cell Signaling Technology, Danvers, MA, United States, 1:1,000 dilution in 1% fat-free milk in TBS-T), anti-phospho-AMPKα (4188, Cell Signaling Technology, Danvers, MA, United States, 1:1,000 dilution in 1% fat-free milk in TBS-T), Article Title: Elevation of p53 sensitizes obese kidney to adriamycin-induced aberrant lipid homeostasis via repressing HNF4α-mediated FGF21 sensitivity. Article Snippet: Proteins were separated by electrophoresis through 10% or 14% SDS-PAGE gels and transferred onto nitrocellulose membranes (GE Healthcare, Amersham, UK). .. The membranes were incubated with anti-NRF2 (1:1000, Abcam), anti-Kelch-like ECH-associated protein 1 (KEAP1), anti-HO-1 (1:1000, Proteintech), anti-NQO1 (1:1000, Santa Cruz), antiSREBP-1c (1:1000, Affinity Biosciences, Cincinnati, OH, USA), anti-PPARα (1:1000, Affinity Biosciences), anti-p53 (1:1000, Proteintech), anti-FGF21 (1:1000, Abcam), anti-phosphorylated extracellular regulated protein kinases (p-ERK, 1:1000, Cell Signaling Technology), anti-ERK (1:1000, Cell Signaling Technology), anti-FGFR1 (1:1000, Cell Signaling Technology), anti-p-FGFR1 (1:1000, Abcam), anti-β-Klotho (KLB, 1:1000, ABclonal Technology), anti-hepatocyte nuclear factor alpha (HNF4α, 1:1000, Abcam), Article Title: MicroRNA-874-3p promotes testosterone-induced granulosa cell apoptosis by suppressing HDAC1-mediated p53 deacetylation Article Snippet: Proteins were separated via 10% SDS-PAGE and transferred to PVDF membranes (EMD Millipore). .. Following blocking with 5% non-fat milk at room temperature for 1 h, the membranes were incubated at 4 ̊C overnight with the following primary antibodies: Anti-androgen receptor (AR; cat. no. 5153; 1:1,000; Cell Signaling Technology, Inc.); Anti-p53 (cat. no. ab26; 1:1,000; Abcam), anti-acetylated p53 (Lys379; cat. no. 2570; 1:1,000; Cell Signaling Technology, Inc.), anti-HDAC1 (cat. no. 34589; 1:1,000; Cell Signaling Technology, Inc.), Article Title: Gastrodiae rhizoma attenuates brain aging via promoting neuritogenesis and neurodifferentiation. Article Snippet: Background: Gastrodiae Rhizoma (Tianma), the dried tuber of Gastrodia elata Bl. (Orchidaceae), is listed as a topgrade herbal medicine in Shen-nong Ben-ts’ao Jing and has been used for treating headaches, dizziness, vertigo and convulsion.. It has a neuroprotective effect and extends the lifespan in mouse models of Huntington’s disease and Niemann-Pick type C disease.. However, its effect on senescence remains unknown. Article Title: Elevation of p53 sensitizes obese kidney to adriamycin-induced aberrant lipid homeostasis via repressing HNF4α-mediated FGF21 sensitivity Article Snippet: Proteins were separated by electrophoresis through 10 % or 14 % SDS-PAGE gels and transferred onto nitrocellulose membranes (GE Healthcare, Amersham, UK). .. The membranes were incubated with anti-NRF2 (1:1000, Abcam), anti-Kelch-like ECH-associated protein 1 (KEAP1), anti-HO-1 (1:1000, Proteintech), anti-NQO1 (1:1000, Santa Cruz), anti-SREBP-1c (1:1000, Affinity Biosciences, Cincinnati, OH, USA), anti-PPARα (1:1000, Affinity Biosciences), anti-p53 (1:1000, Proteintech), anti-FGF21 (1:1000, Abcam), anti-phosphorylated extracellular regulated protein kinases (p-ERK, 1:1000, Cell Signaling Technology), anti-ERK (1:1000, Cell Signaling Technology), anti-FGFR1 (1:1000, Cell Signaling Technology), anti-p-FGFR1 (1:1000, Abcam), anti-β-Klotho (KLB, 1:1000, ABclonal Technology), anti-hepatocyte nuclear factor 4 alpha (HNF4α, 1:1000, Abcam), Pyrolysis Gas Chromatography:Article Title: Effects of American wild ginseng and Korean cultivated wild ginseng pharmacopuncture extracts on the regulation of C2C12 myoblasts differentiation through AMPK and PI3K/Akt/mTOR signaling pathway Article Snippet: .. The membranes underwent incubation with primary antibodies anti-peroxisome proliferator-activated receptor-γ coactivator-1-α (PGC-1α, 1:1,000; cat. no. NBP1-04676; Bioss Antibodies), anti-mitochondrial transcription factor A (TFAM, 1:1,000; cat. no. PA5-68789; Thermo Fisher Scientific, Inc.), anti-nuclear respiratory factor-1 (NRF-1, 1:1,000; cat. no. 69432s; Cell Signaling Technology, Inc.), anti-phospho-AMPK (1:500; cat. no. 44-11509; Thermo Fisher Scientific, Inc.), anti-AMPK (1:500; cat. no. AHO1332; Thermo Fisher Scientific, Inc.), Blocking Assay:Article Title: MicroRNA-874-3p promotes testosterone-induced granulosa cell apoptosis by suppressing HDAC1-mediated p53 deacetylation Article Snippet: Proteins were separated via 10% SDS-PAGE and transferred to PVDF membranes (EMD Millipore). .. Following blocking with 5% non-fat milk at room temperature for 1 h, the membranes were incubated at 4 ̊C overnight with the following primary antibodies: Anti-androgen receptor (AR; cat. no. 5153; 1:1,000; Cell Signaling Technology, Inc.); Anti-p53 (cat. no. ab26; 1:1,000; Abcam), anti-acetylated p53 (Lys379; cat. no. 2570; 1:1,000; Cell Signaling Technology, Inc.), anti-HDAC1 (cat. no. 34589; 1:1,000; Cell Signaling Technology, Inc.), |
